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The side chains of the Glu, Try, and Asp residues form a highly nucleophilic region in the enzyme’s active center, where oxidation of the active site selenocysteine (RSeH) or cysteine (RSH) occurs after binding the peroxide

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Briefly, virion DNA was obtained from the culture medium, capsid-associated core DNA was extracted from the cytoplasmic fraction and the cccDNA was isolated from the nuclear fraction with subsequent plasmid-safe DNase digestion (Epicentre, Madison, WI)